Molecular genetic aspects of microbial biofilm formation. Biofilms are adherent populations of microbes imbedded in a polymeric matrix and adhering to a surface. Biofilm diseases are highly prevalent in humans and eradication of biofilms is a major challenge. We are using a Functional Genomics approach to identify genes that are essential for the formation of pathogenic biofilms by bacteria. Using genome sequence information and DNA microarrays, we have identified a variety of genes of as-yet-unknown function that are essential for maturation of biofilms and tolerance of environmental stress. This project involves a combination of recombinant DNA methodology, biochemistry, physiology and microbiology to understand how bacteria form structurally complex biofilms and to identify new targets for combating infectious agents.

Requirements: Courses in microbiology and molecular genetics are desirable.

Time Commitment: 15-20h/week